ABSTRACT
Background and purpose: Antioxidants and vitamin D3 are currently used for the treatment of neurodegenerative diseases although their mechanism of action is not well understood. The present study was conducted to investigate the effect of combined administration of vitamins D3 and E on demyelination, cell death and remyelination of rat hippocampus following the local ethidium bromide [EB] injection
Methods and Materials: This experimental study was conducted on 32 Spague rats. After EB-induced demyelination, animals received intraperitoneal vitamin E [100 mg/kg] and D3 [5microg/kg] together for 7 days. The extent and intensity of demyelination were studied by luxol fats blue staining, the activated caspase-3 genes and MBP. The study data were analyzed in SPSS using one-way ANOVA and Tukey post test
Results: The findings revealed that the combined administration of vitamins E and D3 for 7 days caused a significant reduction in the expression of activated caspase-3 [10 +/- 0] [p<0.001], as well as a significant increase in MBP expression [236 +/- 30] [p<0.001]. EB injection alone significantly increased demylination [p<0.05]. Combined administration of the two vitamins significantly reduced the extent of demyelination [0.1 +/- 0.03] [p<0.05], and increased remyelination intensity [0.6 +/- 0.06] [p<0.05]
Conclusion: The results of the present study indicated that the combined administration of vitamins E and D3 reduced EB induced demyelination and apopthosis, and improved remyelination
ABSTRACT
Unlimited self renewal and potential capacity of embryonic stem cells [ESCs] in differentiating into a wide variety of cell types has made the cells an attractive source of donor cells for developmental studies and cell therapy. The aim of the present study was the evaluation of the transfection efficiency of pIRES2-EGFP and pcDNA3-hBDNF-v5 plasmids in CCE ES cells by the electroporation method. The plasmids transformed into DH5
Subject(s)
Plasmids , Transfection , Electroporation , Brain-Derived Neurotrophic Factor , Green Fluorescent ProteinsABSTRACT
The present study aimed at evaluating the effect of local hyperthermia on the healing of burn wounds. Right and Left flunks of 8 Balb-c mice [as treated and control wounds respectively] were burned. Local hyperthermia was applied only for the burn wounds of the right flunks [the treated wound]. Sampling was accomplished on the 6[th] day for half of the mice and on the 9[th] day for the other half. The treated wounds had significantly smaller sizes than control wounds on the 6[th] day [P=0.019] and the 9[th] day [P=0.007]. The number of hair follicles and sebaceous glands of the treated wounds were significantly more than those of control wounds both on the 6[th] day [P=0.025 and P=0.043, respectively] and on the 9[th] day [P=0.012 and P=0.033, respectively]. Regarding the neovascularization there was no significant difference between the treated and control wounds on the 6[th] day but on the 9[th] day the neovascularization of the treated wounds was significantly more comparing to the control wounds [P=0.025]. Acute inflammation of the treated wounds was significantly less than the control wounds [P=0.015]. Collagen formation and reepithelialization in the treated wounds were more than the control wounds both on the 6[th] and 9[th] days. It is concluded that local hyperthermia can accelerate the healing process of the second degree burn wounds